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| Gene | Primer sequence (5′–3′) | AT (°C) | PS (bp) |
|---|---|---|---|
| Oct-4 | F-TCAGCTTGGGCTAGAGAAGG R-TGACGGGAACAGAGGGAAAG | 59 | 254 |
| Rex-1 | F-AGCAGGATCGCCTCACTG R-GGCCACTTGTCTTTGCCG | 60 | 189 |
| α-MHC | F-GCGCATCAAGGAGCTCAC R-GGAACTTGGACAGGTTGGTG | 59 | 155 |
| Ncx1 | F-CAGCTTCCAAAACTGAAATCGA R-GTCCCTCTCATCGACTTCCAAAA | 60 | 101 |
| GAPDH | F-GTGGCAAAGTGGAGATTGTTG R-CTCCTGGAAGATGGTGATGG | 60 | 164 |
F, forward; R, reverse; AT, annealing temperature; PS, product size.
