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. 2014 Jun 30;15(1):73. doi: 10.1186/1465-9921-15-73

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Copyright © 2014 Le et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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The inflammatory response in the lungs observed after continuous expose to HDM. Bronchoalveolar lavage fluid (BALF) cells differentiation (A). Eosinophils, neutrophils, lymphocytes and macrophages in BALF demonstrated inflammation in the lungs of HDM-exposed mice, in comparison with control mice. Results are expressed as mean ± SEM. **P < 0.01, ***P < 0.001 (unpaired two-tailed t-test). Representative photomicrographs of H&E (B,D,G) and PAS (C,E,F) stained lung sections. The lung sections from HDM-sensitised and challenged mice showed an infiltration of mononuclear cells (B) and a highly mucus secretion (C) in the airways, whereas the lungs of the control mice do not contain aggregated inflammatory cells (D) and mucus secretion (E). F and G: larger magnifications of C and E. Scale bars of B and D: 200 μm, scale bars of C and E: 100 μm.