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. 2014 Jun 30;15(1):73. doi: 10.1186/1465-9921-15-73

Table 1.

List of antibodies used in this study

Antibody Source Dilution
Primary purified antibodies
Rabbit polyclonal antibody against mouse, human PGP 9.5
Abcam, Cambridge, UK
1:200
Rat monoclonal antibody against mouse I-A/I-E
BioLegend, San Diego, USA
1:200
Goat polyclonal antibody against mouse CGRP
Acris, Herford, Germany
1:400
Armenian hamster monoclonal antibody against mouse CD11c
Abcam, Cambridge, UK
1:100
Armenian hamster monoclonal antibody against mouse CD103
eBioscience, San Diego, USA
1:100
Rat monoclonal antibody against mouse CD11b
eBioscience, San Diego, USA
1:100
Rat monoclonal antibody against mouse F4/80
eBioscience, San Diego, USA
1:100
Rabbit polyclonal antibody against mouse glutamine synthetase (GS)
Abcam, Cambridge, UK
1:500
Rabbit Rabbit polyclonal antibody against Iba1
Wako Chemicals, Japan
1:300
Goat polyclonal antibody against GFAP
Abcam, Cambridge, UK
1:200
Isotype control purified antibodies
Rabbit IgG
Dianova, Hamburg, Germany
1:5000
Rat IgG 2b kappa
BioLegend, San Diego, USA
1:200
Goat IgG
R&D systems,
1:300
Armenian Hamster IgG
BioLegend, San Diego, USA
1:200
Secondary antibodies
DyLight 488 conjugated Donkey Anti-Rabbit IgG antibody
Jackson ImmunoResearch, INC., Baltimore, USA
1:400
Cy3 conjugated Donkey Anti-Rat IgG antibody
Jackson ImmunoResearch, INC., Baltimore, USA
1:500
Cy3 conjugated Donkey Anti-Rabbit IgG antibody
Jackson ImmunoResearch, INC., Baltimore, USA
1:400
DyLight 488 conjugated Donkey Anti-Goat IgG antibody
Jackson ImmunoResearch, INC., Baltimore, USA
1:400
Dylight 649 conjugated Goat Anti-Armenian Hamster IgG antibody
Jackson ImmunoResearch, INC., Baltimore, USA
1:400
DyLight 649 conjugated Donkey Anti-Rabbit IgG antibody
Jackson ImmunoResearch, INC., Baltimore, USA
1:400
Cy3 Conjugated Donkey Anti-Goat IgG
Jackson ImmunoResearch, INC., Baltimore, USA
1:500
Cy5 conjugated Donkey Anti-Rat IgG antibody Jackson ImmunoResearch, INC., Baltimore, USA 1:400