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Immunolocalization of Izumo1 in human sperm. In the upper panel, there are four human sperm, two are acrosome-reacted (shown by arrowhead), and two have acrosome-intact. Izumo1 antibody reacts with only acrosome-reacted sperm and not with acrosome-intact sperm (lower panel). Modified with permission from Inoue et al.21
