Figure 4.

Viral particle structure, cellular recognition, viral proteins, and viral genomic RNA are retained in minimally UV-inactivated virus (MG1-UV^2min). Electron Microscopy analysis of (a) purified virus and (b) 1 × 10⁶ B16lacZ cells infected with virus (500 MOI). Scale bar: 1 cm = value indicated on EM figure; EC, extracellular space; IC, intracellular space. Western blot analysis of viral proteins from (c) 1 × 10⁶ PFU purified virus preparations and (d) 2 × 10⁵ B16lacZ infected with indicated virus (3 MOI). Cells were harvested at 18 hours postinjection; anti-rhabdovirus and GAPDH antibodies were used. Quantitative reverse transcription–PCR analysis of extracted genomic RNA from (e) purified virus preparations and (f) B16lacZ cells infected with virus at 6, 12, and 24 hours postinjection.