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. 2014 May 6;22(7):1320–1332. doi: 10.1038/mt.2014.60

Figure 6.

Figure 6

Maraba MG1 activates NK cell function through conventional dendritic cells. (a) In vitro NK cytotoxicity following NK cell-cDC coculture (^^^P < 0.001 comparing NK cells + DC + MG1 to NK cells + MG1). (b) In vitro infection and (c) activation by flow cytometry of cDC with indicated virus. (d) Quantification of lung tumor metastases at day 3 from B6 mice treated with live or attenuated MG1 i.v. at doses ranging from 1 × 105–7 PFU. Virus was administered at day −1. At day 0, all mice received 3 × 105 B16lacZ tumor cell inoculation via tail vein. (e) Electron microscopy analysis of 1 × 106 bone marrow–derived DCs infected with virus (500 MOI). Scale bar: 1 cm = value indicated on EM figure; EC, extracellular space; IC, intracellular space. (f) PBS or DT was injected intraperitoneally into CD11c-DTR Tg mice at day 0. Twenty-four hours following, MG1 and B16lacZ cells were injected i.v. Spleens were harvested at day 3 to assess ex vivo NK cytotoxicity (^^^P < 0.001 comparing MG1 treatment to PBS and MG1 + DT controls; the data are displayed as the mean percent (±SD) of chromium release from triplicate wells for the indicated E:T ratios). Data are representative of three similar experiments with n = 5–6/group (*P < 0.05).