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. 2014 Apr 29;22(7):1388–1395. doi: 10.1038/mt.2014.50

Figure 3.

Figure 3

In vitro functions of Tregs and natural killer (NK) cells after ultra-low dose (ULD) IL-2. (a) Tcons (CD3+CD4+CD25lowCD127high) equally expressed CD154 through anti-CD3/CD28-coated bead stimulation in pre- and post-IL-2 samples (CD154 expression 52.3 ± 17.5% pre-IL-2 Tcons versus 52.8 ± 17.9% post-IL-2 Tcon; P = 0.86). (b) The suppressive capacity of Tregs on CD154 activation of autologous Tcons was significantly increased in post-IL-2 samples (% suppression 14.0 ± 6.9% pre-IL-2 versus 19.9 ± 8.8% post-IL-2 at Tregs:Tcons ratio 1:1; P = 0.02). (c) Significantly increased interferon (IFN)-γ productions in post-IL-2 NK cells was observed compared to pre-IL-2 NK cells especially in the CD56brightNK cell subset (mean %IFN-γ production 12.0 ± 2.1% pre-IL-2 versus 21.0 ± 4.2% post-IL-2 with IL-12+IL15 and K562 stimulation; P = 0.009). (d) CD107a expression was not significantly different pre- versus post-IL-2 injections (mean %CD107a expression in CD56dimNK cells, 9.6 ± 3.6% pre-IL-2 versus 9.3 ± 3.7% post-IL-2 with IL-12+IL15 and K562 stimulation; P = 0.75).