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. 2014 Mar 25;22(7):1364–1374. doi: 10.1038/mt.2014.33

Figure 6.

Figure 6

M2e antibodies and a modified pattern of IgG isotypes to vaccine are maintained for over a year. BALB/c mice (N = 4) were immunized with influenza split vaccine alone (Split) or influenza split vaccine supplemented with M2e5x VLPs (Split+M2e5x). Long-term antibody responses were determined in immune sera collected at 12 months after boost vaccination. (a) M2e-specific IgG antibody responses. (b) IgG antibody responses reactive to A/California/04/09 (H1N1) virus. (c,d) IgG1 and IgG2a isotype antibodies were determined using influenza A virus, A/California/04/09 (H1N1) as an ELISA coating antigen. (c) IgG isotypes in split vaccine alone. (d) IgG isotypes in split vaccine supplemented with M2e5x VLP. (e) Ratios of IgG2a/IgG1 isotype antibodies. P-value indicates significant difference between split+M2e5x and split vaccinated groups. Error bars indicate mean ± SEM. ELISA, enzyme-linked immunosorbent assay; M2e, M2 ectodomain; VLP, virus-like particles.