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. 2014 Mar 25;22(7):1364–1374. doi: 10.1038/mt.2014.33

Figure 9.

Figure 9

Increased M2e-specific IgG and IgA antibodies in BALF are maintained by M2e5x VLP-supplemented vaccination for over a year. Levels of IgG and IgA antibodies were determined day 4 postchallenge (N = 4) from the mice at the time of 12 months after boost vaccination. (a) IgG antibody level to A/California/04/09 (H1N1) virus. (b) M2e-specific IgG antibody response. (c) IgA antibody level to A/California/04/09 (H1N1) virus. (d) M2e-specific IgA antibody response. IgG or IgA antibody responses were determined by ELISA using inactivated influenza virus or human type M2e peptide as a coating antigen (4 μg/ml). P value indicates significant difference between split+M2e5x and split vaccinated or naive groups. Error bars indicate mean ± SEM. BALF, bronchoalveolar lavage fluid; ELISA, enzyme-linked immunosorbent assay; M2e, M2 ectodomain; pg, pictogram; VLP, virus-like particles.