Table 1. RNA markers and primer sequences used for human tissue identification in the 18 mRNA markers.
| Gene | Body fluids | [primer] µM Single/Multi | Genebank accession number | Primer sequence (5′to3′) | Size(bp) | Reference |
| GD | Circulatory blood | 0.4/0.2 | NM_000190 | fw: TGG ATC CCT GAG GAG GGC AGA AG rv: TCT TGT CCC CTG TGG TGG ACA TAG CAA T | 177 | [11] |
| LY | Circulatory blood | 0.4/0.3 | NM_002099 | fw: CAG ACA AAT GAT ACG CAC AAA CG rv: CCA ATA ACA CCA GCC ATC ACC | 188 | [14] |
| HBB | Circulatory blood | 0.1/0.04 | NM_000518 | fw: CTG AGA ACT TCA GGC TCC TGG G rv: CAG CAA GAA AGC GAG CTT AGT G | 159 | * |
| TN3 | Saliva | 0.2/0.15 | NM_000200 | fw: TCA CAT CGA GGC TAT AGA TCA AA rv: GTG TGA TGC GGT ATG ACA AAT | 134 | * |
| STATH | Nasal secretion | 0.4/0.3 | NM_003154 | fw: ATT GGC CCT CTA GGG TAG CA rv: AGG GCC ATA CCC ATA ACC GA | 213 | * |
| PRM2 | Semen | 0.2/0.15 | NM_002762 | fw: GTG AGG AGC CTG AGC GAA CGC rv: TTA GTG CCT TCT GCA TGT TCT CTT C | 294 | [11] |
| TGM4 | Semen | 0.2/0.15 | NM_003241 | fw: AGC CTG GGC ATC TCC TCA CTA CA rv: TGG GTC CAG TTT TTA TTG GGG TGC | 103 | [15] |
| MUC4 | Vaginal secretion | 0.6/0.4 | NM_138297 | fw: GGA CCA CAT TTT ATC AGG AA rv: TAG AGA AAC AGG GCA TAG GA | 235 | [11] |
| HBD1 | Vaginal secretion | 0.8/0.4 | NM_005218 | fw: AGA TGG CCT CAG GTG GTA AC rv: GTC ACT CCC AGC TCA CTT GC | 170 | * |
| MMP7 | Menstrual blood | 0.05/0.025 | NM_002423 | fw: GAA CAG GCT CAG GAC TAT CTC rv: TAA CAT TCC AGT TAT AGG TAG GCC | 126 | [4] |
| MMP11 | Menstrual blood | 0.6/0.4 | NM_005940 | fw: ACC TTT ACT GAG GTG CAC GAG rv: CAA ATT CAT GGG CTG CCA CC | 223 | * |
| KRT4 | Oral mucosa | 0.05/0.015 | NM_002272 | fw: AAA GTC CGG ACG GAA GAG rv: TAA GAA CTG CAC CTT GTC G | 81 | [4] |
| UMOD | Urine | 0.8/0.5 | NM_003361 | fw: GGG ACA GTG TTG ACG AGG AA rv: ATG AGC AGT GCA AAT CGG GA | 341 | * |
| DCD | Sweat | 0.6/0.4 | NM_053283 | fw: TTT GGT GGC ATA CCC ACT CC rv: CTT TGG TGC CTG TCT GGC TA | 204 | * |
| LOR | Skin | 0.8/0.4 | NM_001264 | fw: CTT TGG GCT CTC CTT CCT rv: AGA GGT CTT CAC GCA GTC | 89 | [4] |
| CST6 | Skin | 0.8/0.4 | NM_001323 | fw: CAC GTC GAC CTC ACC ACT TG rv: GCC CTC GGG GAC TTA TCA CA | 147 | * |
| GAPDH | House-keeping | 0.2/0.2 | NM_001256799 | fw: GTC CAC TGG CGT CTT CAC CA rv: GTG GCA GTG ATG GCA TGG AC | 261 | [4] |
| 18SrRNA | House-keeping | 0.025/0.025 | NR_003286 | fw: CTC AAC ACG GGA AAC CTC AC rv: CGC TCC ACC AAC TAA GAA CG | 110 | [4] |
*Original designed/redesigned for this study using Primer Premier v5.0.