Table 3. Sensitivity of the RNA profiling methods using quantitative approach (input totalRNA) by real-time PCR.
| RNA input [ng] | |||||||
| Body Fluid | Marker | 25 | 5 | 1 | 0.2 | 0.04 | 0.008 |
| Blood | HBB | 10/10 | 10/10 | 10/10 | 10/10 | 10/10 | 10/10 |
| GLY | 10/10 | 10/10 | 10/10 | 7/10 | 2/10 | 0/10 | |
| PBGD | 10/10 | 10/10 | 7/10 | 3/10 | 0/10 | 0/10 | |
| Nasal secretion | STATH | 8/8 | 8/8 | 8/8 | 7/8 | 4/8 | 1/8 |
| Saliva | 8/8 | 8/8 | 8/8 | 6/8 | 3/8 | 0/8 | |
| HTN3 | 8/8 | 8/8 | 8/8 | 8/8 | 7/8 | 5/8 | |
| Semen | PRM2 | 4/4 | 4/4 | 4/4 | 4/4 | 4/4 | 3/4 |
| TGM4 | 6/6 | 6/6 | 6/6 | 6/6 | 6/6 | 5/6 | |
| Vaginal secretion | MUC4 | 5/5 | 5/5 | 5/5 | 4/5 | 2/5 | 0/5 |
| HBD1 | 5/5 | 5/5 | 5/5 | 5/5 | 3/5 | 1/5 | |
| Menstrual blood | MMP7 | 5/5 | 5/5 | 5/5 | 5/5 | 5/5 | 3/5 |
| MMP11 | 5/5 | 5/5 | 5/5 | 5/5 | 3/5 | 1/5 | |
| Oral mucosa | KRT4 | 8/8 | 8/8 | 8/8 | 8/8 | 8/8 | 8/8 |
| Urine | UMOD | NT | NT | 14/16 | 11/16 | 3/16 | 0/16 |
| Sweat | DCD | NT | NT | 6/6 | 5/6 | 2/6 | 1/6 |
| Skin | LOR | NT | NT | 6/6 | 6/6 | 5/6 | 4/6 |
| CST6 | NT | NT | 6/6 | 6/6 | 5/6 | 3/6 | |
Total RNA was extracted from samples from 4–16 donors (as described in 2.2.1). A range of input total RNA was reverse transcribed and the resulting cDNA was amplified. Each experiment was carried out 3 times. dCt≤23 represent positive results. NT: not tested.