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Coomassie-blue-stained gel of the His-Tag-purified CAs from P. aeruginosa PAO1. Lanes: 1, protein ladder; 2 and 3, psCA1 boiled and not boiled; 4 and 5, psCA2 boiled and not boiled; 6 and 7, psCA3 boiled and not boiled. Arrowheads show protein dimerization. The identity of the proteins was confirmed by MALDI-TOF.
