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Immunoblot analysis of cytosolic cell extract from P. aeruginosa PAO1 grown in BMM at ambient air or 5 % CO2. Lanes: 1, protein ladder; 2, PAO1 cell extract at ambient air; 3, PAO1 cell extract at 5 % CO2; 4, 5 and 6, purified psCA1, psCA2 and psCA3, respectively. The CAs were probed with the primary antiserum raised against β-class CA, Cab from Me. thermoautotrophicum (gift from Dr James Ferry, Pennsylvania State University; Smith et al., 1999).
