Growth characteristics of strain PAO1 and transposon mutants PW1172, PW4542 and PW8872 with the corresponding genotypes: PAO102-E10 : : ISphoA/hah, PA2053-H07 : : ISphoA/hah and PA4676-B07 : : ISlacZ/hah, obtained from the University of Washington Two-Allele library. The cultures were grown in BMM in ambient air (a), or at 5 % CO2 (b). To inhibit CA activity, 100 µM AZ in 50 % DMSO was added. All graphs represent the means of three independent growth experiments.