Table 1. PCR primers used in this study.
Primer | Sequence (5′→3′) | Comments |
PAO102_F* | AGAGAGCATATGCCAGACCGTATG | External psCA1 primer, forward |
PAO102_R | AGAGAGGGATCCTCACGAGCTCAG | External psCA1 primer, reverse |
PA2053_F | AGAGAGCATATGCGTGACATCATCG | External psCA2 primer, forward |
PA2053_R | AGAGAGGGATCCTCAGGCGAC | External psCA2 primer, reverse |
PA4676_F | AGAGAGCATATGAGCGACTTGCAG | External psCA3 primer, forward |
PA4676_R | AGAGAGGGATCCTCAGCAGCAAC | External psCA3 primer, reverse |
The forward and the reverse primers include the recognition sites for NdeI and BamHI (underlined). Six bases (AGAGAG) were added to the start of each primer.