Skip to main content
. 2013 Aug;159(Pt 8):1748–1759. doi: 10.1099/mic.0.066357-0

Table 1. PCR primers used in this study.

Primer Sequence (5′→3′) Comments
PAO102_F* AGAGAGCATATGCCAGACCGTATG External psCA1 primer, forward
PAO102_R AGAGAGGGATCCTCACGAGCTCAG External psCA1 primer, reverse
PA2053_F AGAGAGCATATGCGTGACATCATCG External psCA2 primer, forward
PA2053_R AGAGAGGGATCCTCAGGCGAC External psCA2 primer, reverse
PA4676_F AGAGAGCATATGAGCGACTTGCAG External psCA3 primer, forward
PA4676_R AGAGAGGGATCCTCAGCAGCAAC External psCA3 primer, reverse
*

The forward and the reverse primers include the recognition sites for NdeI and BamHI (underlined). Six bases (AGAGAG) were added to the start of each primer.