FIG. 3.

PLA₂ and 12/15-LOX are involved in regulation of EAEC 042-induced PMN transepithelial migration. A and C: T84 monolayers were pretreated with the pan-PLA₂ inhibitor ONO-RS-082 for 3 hours (A) or with the 12/15-LOX inhibitor baicalein for 48 hours (C) and assessed for effect on EAEC 042-induced PMN transepithelial migration. fMLP was included as a positive control. B and D: Monolayers of HCT-8 cells transfected with a vector control or a vector modified to generate siRNAs aimed at decreasing the expression of PLA₂G6 (B) or ALOX15 (D) were assessed for their effect on EAEC 042- or E. coli F-18-induced PMN transepithelial migration. The data are expressed as the mean ± SD for triplicate samples and represent one of at least three independent experiments performed with similar results. ***, p < 0.001; **, p < 0.01.