FIG. 4.

Activation of the 12/15-LOX pathway after infection with EAEC 042 as demonstrated by quantification of arachidonic acid metabolites 12-S-HETE, 15-S-HETE, LTB₄, and PGE₂. ELISA's were performed on cell extracts collected from T84 cells (see Experimental procedures). Data represents the ratio of each metabolite from 042 infected samples to a baseline (set to one) from negative HBSS⁺ controls. Data are expressed as mean ± SD of duplicate samples from at least three experiments performed. Raw means of each 042 infected metabolite are as follows, 12-S-HETE: 25.2 ng, 15-S-HETE: 16.5 ng, LTB₄: 0.232 ng, PGE₂: 0.216 ng.