FIG. 6.

EAEC 042 induces secretion of PMN-chemoattractive bioactive lipids through MRP2. T84 cell monolayers grown on plastic flasks were pretreated with HBSS⁺ or 100 μM probenecid (Prob) for 24 hours and then infected with EAEC 042. Uninfected cells served as a negative control (-). Following infection, crude preparations collected from cell culture medium of the monolayers were collected and lipids present in the media extracted from it. The lipids were then added to the apical chambers of polarized T84 cells monolayers and assessed for their ability to induce PMN transepithelial migration. The data are expressed as the mean ± SD for triplicate samples and represent one of at least three independent experiments performed with similar results. ***, p < 0.001.