Skip to main content
. Author manuscript; available in PMC: 2015 Apr 1.
Published in final edited form as: Microbes Infect. 2013 Dec 25;16(4):300–309. doi: 10.1016/j.micinf.2013.12.003

Fig. 4. Schu S4 strains lacking the gcvT homolog have lower bacterial burdens at distal sites.

Fig. 4

Overnight TSB-C broth cultures of Schu S4 strains were pelleted, resuspended in PBS, and intratracheally administered to anesthetized mice at 100 CFU. Four days post infection, the lung (A), spleen (B), liver (C) and blood (D) were harvested, homogenized, and plated to enumerate CFU. Data in each panel are derived from four experiments: two independent experiments compared Schu S4 to Schu S4 ΔgcvT and two independent experiments compared Schu S4 ΔgcvT:pMB1gcvT to Schu S4 ΔgcvT:pMB1. These groups of experiments are separated by a dotted line. Each symbol denotes the CFU from a single mouse. Each strain had at least four mice per experiment and at least eight mice total. A * denotes statistical significance (p < 0.05) by two-sided t-test. The limit of detection was 50 CFU for the lung, 100 CFU for the spleen and blood, and 200 CFU for the liver.