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. Author manuscript; available in PMC: 2015 Apr 1.
Published in final edited form as: Microbes Infect. 2013 Dec 25;16(4):300–309. doi: 10.1016/j.micinf.2013.12.003

Fig. 5. A functional glycine cleavage system is required for robust intracellular replication using minimal media conditions.

Fig. 5

Overnight TSB-C broth cultures of Schu S4 strains were pelleted, resuspended in PBS, and added to mouse macrophages at an MOI of 500. Bacteria were cocultured with cells for two hours and subsequently subjected to a gentamicin protection assay. Following the gentamicin treatment, minimal media or minimal media with 25 mM serine was added instead of standard tissue culture media. Strains were already confirmed in Fig. 2D to not have any differences in invasion (two hour time point) under the same conditions used in this assay. Cells were lysed 24 hours post infection and CFU was enumerated. Data are expressed as the mean ± SEM of three independent experiments. Significant difference (p < 0.05) are denoted by a * and were assessed using one-way ANOVA followed by a Bonferroni multiple comparison correction.