Fig. 5.
Effect of γ-globulin therapy on anti-Candida albicans solubilized cell wall glucan (CSBG) titre in Kawasaki disease patients. The enzyme-linked immunosorbent assay (ELISA) plate was coated with CSBG. The sera were added to the plate, and the plate-bound immunoglobulin (Ig) was determined with peroxidase-conjugated anti-human IgG+M+A. Enzyme activity was measured by the addition of 3,3′,5,5′-tetramethylbenzidine (TMB) substrate. Pre = Kawasaki disease patients hospitalized (n = 18); post = Kawasaki disease patients after γ-globulin therapy (n = 18); post 1 month = Kawasaki disease patients 1 month after γ-globulin therapy (n = 18).