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Nitric oxide (NO) and superoxide (O2−) expression in polymorphonuclear leucocytes (PMNs) in sputum from cystic fibrosis (CF) patients with chronic Pseudomonas aeruginosa lung infection. Visualization of NO and O2− production in PMNs in sputum by fluorescence microscopy stained with 20 μM 4-amino-5-methylamino-2′,7′-difluorofluorescein diacetate (DAF-FM) (green colour) and 5 μM hydroethidine (red colour).
