Fig. 1.

Increased numbers of Gr-1⁺ granulocytes (a), F4/80⁺ macrophages/monocytes (b), dendritic cells (DCs) (c) and CD4⁻CD8⁻ T cells (d) in peripheral blood (PB) of [C57BL/6 × spontaneous crescentic glomerulonephritis-forming/Kinjoh (SCG/Kj)] F₂ intercross (BSF₂) mice with glomerulonephritis (GN). Not plasmacytoid (CD11c⁺B220⁺) but conventional (CD11c⁺B220⁻) DCs increased in GN (+) mice (e,f). Frequencies of each leucocyte in PB were counted using flow cytometry in C57BL/6 (B6), (C57BL/6 × SCG/Kj) F₁ (BSF₁), BSF₂ without GN [GN (−)] and BSF₂ with GN [GN (+)] mice at 12 weeks of age. Numbers of each leucocyte were calculated by multiplying the frequency and the whole white blood cell number. Whole leucocyte gates (blood cells except red blood cells and platelets) were examined. For CD4⁻CD8⁻ T cells, CD3⁺ cells were further gated and analysed. Mean values ± standard error of the mean are shown. P-values were determined with analysis of variance (anova) and Fisher's protected least significant difference procedure. Numbers of mice are shown in parentheses.