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. Author manuscript; available in PMC: 2014 Aug 20.
Published in final edited form as: Biochemistry. 2013 Jul 24;52(33):5656–5664. doi: 10.1021/bi400358v

Figure 5.

Figure 5

Role of W313 and W315 in the digestion of BC by TfCel48A. (a) The tunnel and active site of TfCel48A. W313 and W315 residues are located at the mouth and inside the tunnel respectively; (b) time course profiles of WT, W313A, and W315A on BC (N=3), fitted with Eqn. 6; (c) apparent KM experiments with WT, W313A, and W315A on BC; (d) Eqn. 6 parameter values, processivity, and apparent KM of WT and mutated TfCel48A. Processivity is calculated as a ratio of cellobiose (G2) to cellotriose (G3) (33).