Table 1.

A and b values of all T. fusca cellulases digestion time course profiles on BC.

Enzyme*	Endo/ Exo	A# (% digestion hr−1)	b#	Processivity%
TfCel9A	Endo	1.68 ± 0.04	0.58 ± 0.02	6.9
TfCel9A-CD	Endo	0.90 ± 0.01	0.58 ± 0.01	2.5–3.5
TfCel5A	Endo	1.61 ± 0.02	0.33 ± 0.02	N/A
TfCel9B	Endo	1.11 ± 0.03	0.31 ± 0.01	N/A
TfCel6A	Endo	1.18 ± 0.07	0.38 ± 0.01	N/A
TfCel6B	Exo	0.48 ± 0.01	0.50 ± 0.01	20
TfCel48A	Exo	0.52 ± 0.01	0.33 ± 0.01	15
TfCel48A-CD	Exo	0.24 ± 0.01	0.33 ± 0.01	15
T. fusca crude supernatant	N/A	13.97 ± 0.23	0.69 ± 0.01	N/A
T. reesei crude supernatant	N/A	15.32 ± 0.22	0.64 ± 0.01	N/A

^*Individual cellulase concentrations, except TfCel48A, were 33.3 nM; TfCel48A and TfCel48A-CD concentrations were 100 nM; T. fusca and T. reesei crude supernatant total protein added per reaction was 95 µg and 8 µg respectively.

^#N = 3 for all data points. Averaged values with standard deviations were fit to Equation 6 to obtain A and b values. Standard error is provided for all values. All R² > 0.97.

^%Values for TfCel9A are the ratio of soluble and insoluble reducing ends after digestion of filter paper and were previously published (20, 42). Values for TfCel6B and TfCel48A are the ratio of cellobiose to cellotriose concentrations (33) after digestion of BC.

N/A = not applicable.