Fig. 1.

Presence of A_2BR and A₃R in P6 and P30 LAL muscle. (A) Representative Western blotting analysis of A_2BR (top) and A₃R (bottom) (with antibodies from Millipore and Santa Cruz) protein presence in lysates of adult muscle (P30, line 1), neonatal muscle (P6, line 2), adult brain (line 3) and adult spinal cord (line 4). Samples are 100 μg of protein. Line 5 (for A_2BR and A₃R from Millipore) is the negative control, incubated with blocked peptide. Line 5 (for A₃R from Santa Cruz) is the negative control incubated without primary antibody). As a positive control, we used adult brain and spinal cord. Actin immunoblots were used for protein loading controls (not shown). (B) Quantitative analysis of several A_2BR (top) and A₃R (bottom) Western blots normalized by actin staining. The data show that the presence of A_2BR and A₃R protein is more abundant in the adult muscle than in the newborn muscle when both antibodies are used. Also, A_2BR is more abundant in brain and spinal cord than in muscle whereas A₃R protein is slightly more abundant in muscle than in brain and spinal cord. Results are expressed as mean ± SEM of five independent experiments, *P < 0.05.