For 500 ml of DMEM/F12
Stock solution	Volume	final concentration
N2 supplement (10x)	5 ml	1x
250 mg/ml BSA stock solution	1 ml	0.5 mg/ml
10 mg/ml Gentamicin reagent	1.25 ml	25 μg/ml
100x antibiotic/antimycotic	2.5 ml	0.5x
100 mg/ml bFGF	0.1 ml	20 ng/ml
100 mg/ml EGF	0.1 ml	20 ng/ml