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. 2014 Jul 8;55(7):4175–4185. doi: 10.1167/iovs.13-12856

Figure 3.

Figure 3

Aminoimidazole carboxamide ribonucleotide treatment of uveal melanoma cells is associated with activation of AMPK. (A) Western blot analysis of phosphorylated ACC (Ser-79) expression in 92.1, MEL 270, and MEL 202 cells that were treated with AICAR at a concentration of either 1 or 2 mM for 24 hours. (B) Western blot analysis of phosphorylated ACC expression in 92.1, MEL 270, and MEL 202 cells pretreated with DPY for 30 minutes before addition of AICAR at a concentration of 2 mM for 24 hours. (C) Western blot analysis of phosphorylated ACC expression in 92.1, MEL 270, and MEL 202 cells pretreated with iodo for 30 minutes before addition of AICAR at a concentration of 2 mM for 24 hours. Density values of phosphorylated ACC bands are graphically expressed relative to control. Multiple bands represent separate biological samples. Significance (*) is assigned at P < 0.05.