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. 2014 Jun 26;2:e461. doi: 10.7717/peerj.461

Figure 2. Competition experiments defining structural requirements of heparin derivatives that enable Fc rNRP-1 binding.

Figure 2

Inhibiting ability of oligosaccharides of selected lengths and modified heparin derivatives on Fc rNRP-1 binding to the heparin derivatised surface was tested, as described in the experimental section. (A), (C) The relative binding values at each concentration of added sugar competitor were adjusted to 100% of Fc rNRP-1 binding alone to the immobilised heparin (measured at between 10–40 arc s). In (A) only a selection of curves demonstrating the largest changes in IC50 are shown for clarity, the full set are in Fig. S2). The experiment was performed three times independently (mean ± SD). (B), (D) IC50 values of the compounds from the experiments described in panels A and C were calculated from the non-linear curve fit of the respective binding curves and are expressed in (mean ± SE µg/mL).