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. 2014 Jun 26;2:e461. doi: 10.7717/peerj.461

Figure 5. Sypro stability assay of recombinant NRP-1s in the presence and absence of heparin.

Figure 5

(A) Fc rNRP-1, (B) shNRP-1 and (C) Fc were subjected to denaturation cycle alone or with the indicated amount of heparin in the presence of Sypro Orange dye. The melting curves (mean of three shown) were recorded by the RT-PCR instrument and analysed as described in the Methods. The first derivatives of the melting curves were plotted. (D) The melting temperature (Tm) values equal to the maxima of the derivatives from A, B, C.