Table 2. Kinetics and affinity of Fc rNRP-1 binding to a heparin-derivatised surface in an optical biosensor.
ka is the association rate constant, p value is the correlation coefficient of the linear regression through the kon values, intercept is the kd value obtained from kon plots , kd is the dissociation rate constant obtained in the dissociation experiments in the presence of competing heparin, and Kd is the affinity calculated from the ratio of kd/ka. The standard errors (SE) were calculated from five independent association datasets and six independent dissociation datasets and combined for the calculation of Kd.
| ka (Ms)−1 | p | Intercept (s−1) | kd (s−1) | Kd (nM) |
|---|---|---|---|---|
| 9,800,000 ± 2,100,000 | 0.96 | 0.005 ± 0.0001 | 0.025 ± 0.0007 | 2.5±0.5 |