Table 3. List of peptides identified in the protect and label analysis of the binding sites for heparin in Fc rNRP-1.
Peptide | Mass experimental | Mass calculated | Error (ppm) | Sequence | Residues | Domain |
---|---|---|---|---|---|---|
1 | 1800.96 | 1800.91 | 24.3 | VRIK(Biotin)PASWETGISM | 404–417 | b1 |
2 | 1494.76 | 1494.6464 | 76 | AGAFR(Acetyl)SDK(Biotin)C(Carbamidomethyl)GGT | 19–30 | a1 |
3 | 1926.02 | 1926.0953 | -39.1 | DK(Biotin)NISRKPGNVLKTL(Ammonium) | 822–836 | L2 |
4 | 2024.1 | 2024.0481 | 26.4 | TLPPSRDELTK(Biotin)NQVSL | 978–993 | IgG1 Fc |
5 | 2125.14 | 2125.0059 | 63.1 | VVVDVSHEDPEVK(Biotin)FNW | 890–905 | IgG1 Fc |
6 | 1452.74 | 1452.6358 | 68.5 | AGAFRSDK(Biotin)C(Carbamidomethyl)GGT | 19–30 | a1 |
7 | 1790.91 | 1790.8643 | 26.1 | TVDK(Biotin)SRWQQGNVF | 1039–1051 | IgG1 Fc |
8 | 1381.71 | 1381.6603 | 43.3 | NGK(Biotin)EYKCKVS | 943–951 | IgG1 Fc |
9 | 1516.72 | 1516.7464 | −14.7 | QVIFEGEIGK(Biotin)GN | 778–789 | c |
10 | 1811.92 | 1811.9069 | 7.25 | SQADENQK(Biotin)GK(Acetyl)VARL | 695-708 | c |
11 | 2074.06 | 2074.0109 | 28 | MVVGHQGDHWK(Biotin)EGRVL | 754–769 | c |
12 | 1182.65 | 1182.6452 | 4.05 | VRIK(Biotin)PASW | 404–411 | b1 |
13 | 1909 | 1909.0688 | −36 | DK(Biotin)NISRKPGNVLKTL | 822–836 | L2 |
14 | 1628.7 | 1628.6621 | 23.3 | HSYHPSEK(Biotin)CEW | 46–56 | a1 |
15 | 1364.69 | 1364.6337 | 41.3 | NGK(Biotin)EYKCKVS -NH3 | 943–951 | IgG1 Fc |