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. 2014 May 8;124(6):2550–2559. doi: 10.1172/JCI65928

Figure 3. The pharmacological inhibition of nNOS with l-NAME prevents the rescue of the estrous cycle and LH levels by leptin in fasted mice.

Figure 3

(A) Representative 15-day estrous cycles of animals subjected or not to 24-hour fasting in diestrus 1 three days before death (gray shadow). Mice were subjected to leptin (red arrows), l-NAME (blue arrows), or vehicle (white arrows) injection twice daily on diestrus 1 three days before death. Each circle represents 1 day. Di, diestrus; P, proestrus; E, estrus. (B) Leptin treatment in fasting mice rescued proestrous-like uterine weight (UW), whereas concomitant l-NAME injection blunted this effect. (C) Leptin treatment restores surge levels of LH in mice subjected to 24-hour fasting in diestrus 1 and killed on the expected day of proestrus, i.e., 2 days after. l-NAME treatment impedes this leptin-rescuing effect in fasting mice. *P < 0.05, ***P < 0.001, leptin vs. vehicle.