Figure 5. nNOS deficiency does not alter GnRH expression in Lep^+/+ and Lep^ob/ob mice.

(A) The number of GnRH neurons in the preoptic region and (B and C) the density of GnRH-immunoreactive (ir, green) fibers in the median eminence. Interestingly, the Nos1^+/+ Lep^ob/ob mice showed an increased GnRH immunoreactivity, while that of Nos1^–/– Lep^ob/ob and Nos1^+/+ Lep^ob/ob female mice treated with leptin was equivalent to that of wild-type and Nos1^–/– Lep^+/+ mutant mice. Scale bar: 200 μm. *P < 0.05, **P < 0.01.