Skip to main content
. 2014 May 8;124(6):2523–2537. doi: 10.1172/JCI69557

Figure 6. Knockdown of KLF4 expression caused an increase in albumin permeability in cultured podocytes with ADM treatment.

Figure 6

(A) Representative photomicrographs of (upper panel) cell morphology and (lower panel) immunofluorescence labeling with F-actin in cultured human podocytes transfected with KLF4 siRNA or control RNA for 72 hours. Scale bars: 50 μm (upper panel); 10 μm (lower panel). (B) (Upper panel) Representative Western blots of KLF4 and (lower panel) real-time RT-PCR analysis of mRNA expression in podocytes transfected with KLF4 siRNA or control RNA for 72 hours (n = 6). (C) Real-time RT-PCR analysis of mRNA expression in ADM-treated (0.3 μg/ml) podocytes transfected with KLF4 siRNA or control RNA for 48 hours (n = 6). (D and E) In vitro permeability of FITC-labeled albumin in podocytes transfected with KLF4 siRNA or control RNA without (D) or with (E) ADM (0.3 μg/ml) for 48 hours (n = 6). *P < 0.05; **P < 0.01 vs. controls.