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. 2014 May 16;124(6):2750–2761. doi: 10.1172/JCI74604

Figure 3. Specific knockdown of SPHK1 in HSCs in BMT SCD chimeras reduced erythrocyte SPHK1 protein levels and erythrocyte and plasma S1P levels.

Figure 3

(A) HPLC and electrophoresis analysis of HbS and mouse normal Hb (HbA) in BMT SCD chimeras to assess the percentage of chimerism. Representative electrophoresis analysis and the average of percentage of chimerism with HbS in SCD chimeras were shown as insets. Data shown represent the mean ± SEM (n = 6–9). (B) Erythrocyte SPHK1 levels, (C) activity, (D) plasma S1P levels, and (E) erythrocyte S1P levels were significantly reduced in the SCD chimeras with specific SPHK1 knockdown compared with those in mice with BMCs infected with a scrambled shRNA. *P < 0.05 versus SCD chimeras with BMCs infected with recombinant lentivirus encoding scrambled shrna.