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. 2014 May 16;124(6):2750–2761. doi: 10.1172/JCI74604

Figure 5. Specific knockdown of SPHK1 in HSCs in BMT SCD chimeras reduced splenomegaly and tissue damage.

Figure 5

(A) Spleen size and (B) H&E staining of spleens, livers, and lungs of SCD chimeras with HSC-specific SPHK1 knockdown and controls. (C) Representative Evans blue staining and (D) quantification of its concentration in the lungs of the control SCD chimeras and SCD chimeras with specific SPHK1 knockdown. (E) Albumin concentrations in bronchial alveolar lavage (BAL) fluid collected from the control SCD chimeras and SCD chimeras with specific SPHK1 knockdown. Values shown represent the mean ± SEM (n = 6–11). *P < 0.05 versus SCD chimeras with BMCs infected with recombinant lentivirus encoding scrambled shrna. Scale bar: 200 μM.