Table 2. Binding affinities of different SiaPs to different sugars and entropic and enthalpic components.

MicroCal ITC was used for the measurements and the data were analyzed using the Origin software.

SiaP	Ligand	K d (nM)	H (calmol1)	T S (calmol1)
F. nucleatum	Neu5Ac	45.5	9165	849.3
	Neu5Gc	45.7	12074	2059.4
P. multocida	Neu5Ac	19.7	6560	3948.2
	Neu5Gc	30.7	7469	2777.3
V. cholerae	Neu5Ac	306	7175	508.9
	Neu5Gc	1090	5200	1127.6
H. influenzae †	Neu5Ac	28	15300	5000

^†Thermodynamic data for Hi-SiaP with Neu5Ac are taken from Johnston et al. (2008 ▶) for comparison with other SiaPs.