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MicroCal ITC was used for the measurements and the data were analyzed using the Origin software.
| Hi-SiaP (with Neu5Ac) | K d | H (calmol1) | T S (calmol1) |
|---|---|---|---|
| Wild type | 16.6nM | 17500 | 6883 |
| E67A | 19.8M | 249.5 | 6168.6 |
| E186Q | 1.78M | 6827 | 1016 |
| N187Q | 1.41M | 7939 | 40.826 |
| T64K | 0.641M | 8743 | 294.72 |
| H209A | 0.662M | 13360 | 4917 |
| T64R | No measurable binding | ||
| R127K | No measurable binding | ||
