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. Author manuscript; available in PMC: 2014 Jul 9.
Published in final edited form as: Chem Res Toxicol. 2011 May 13;24(6):855–865. doi: 10.1021/tx200046s

Figure 5.

Figure 5

(−)-Xanthatin suppresses key genes involved in the G2/M checkpoint. (A) GADD45 molecules can inhibit Cdc2/cyclin B1 involved in the G2/M checkpoint. (B-a) Results of DNA microarray analysis were presented. Data are expressed as the fold induction vs vehicle-treated groups. MDA-MB-231 cells were treated with the vehicle or 10 µM (−)-xanthatin for 48 h, followed by mRNA isolation. Details of microarray conditions are described under Materials and Methods. (B-b) RT-PCR analysis of Cdc2 and cyclin B1 mRNA levels after treatment with (+) or without (−) 10 µM (−)-xanthatin. β-Actin was used as an RNA normalization control. Also see the inset (Cdc2). A100-bp DNA ladder marker was also loaded. Xa, (−)-xanthatin. A representative data image is shown.