Table 1. Macromolecule-production information.

The residues in the final protein after tag removal are underlined.

Source organism	Saccharomyces cerevisiae
DNA source	Genomic DNA
Imp3 26–183
Forward primer	GAACAGATTGGTGGATCCGGCCACCGGGACACTCAG
Reverse primer	CGACGGAGCTCGAATTATGAAAAATCAAAATCGTC
Expression vector	pET-28a-His-SMT3
Expression host	Rosetta 2(DE3)
Complete amino-acid sequence of the construct produced	MGSSHHHHHHSSGLVPRGSHMASMSDSEVNQEAKPEVKPEVKPETHINLKVSDGSSEIFFKIKKTTPLRRLMEAFAKRQGKEMDSLRFLYDGIRIQADQTPEDLDMEDNDIIEAHREQIGGSGHRDTQVMRTYHIQNREDYHKYNRICGDIRRLANKLSLLPPTDPFRRKHEQLLLDKLYAMGVLTTKSKISDLENKVTVSAICRRRLPVIMHRLKMAETIQDAVKFIEQGHVRVGPNLINDPAYLVTRNMEDYVTWVDNSKIKKTLLRYRNQIDDFDFS
Mpp10 430–461
Forward primer	AAGTTCTGTTCCAGGGGCCCCTTCAAAAGGCACATTCC
Reverse primer	GCCGCAAGCTGAATTAAGGTTGAGCATCCTCCAT
Expression vector	pETDuet-His-GST
Expression host	Rosetta 2(DE3)
Complete amino-acid sequence of the construct produced	MGSSHHHHHHSQDPMSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPKSDLEVLFQGPLQKAHSEISELYANLVYKLDVLSSVHFVPKPA