Figure 2.
Purification, characterization and crystallization of purified carboxy-terminal parts of bacteriophage T4 proximal long tail fibre protein gp34. (a) Denaturing gel electrophoresis of gp34(894–1289). The elution fractions 1–8 of the purification by strong anion-exchange chromatography are shown; molecular-weight markers are in lane M (labelled in kDa). (b) Protease digestion of gp34(726–1289) with nondigested protein in lane 1, once digested protein in lane 2, twice digested in lane 3 and three times digested in lane 4. Molecular-weight markers are shown in lane M (labelled in kDa). (c) Transmission electron microscopy of gp34(894–1289). The scale bar represents 50 nm. (d) Crystals of gp34(894–1289) of the H32 form. (e) Crystals of gp34(894–1289) of the P21 form. (f) Crystals of gp34(726–1289). (g) Crystals of selenomethionine-derivatized gp34(781–1289).