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Diffraction images from cryocooled and room-temperature diffraction experiments. (a) Diffraction image from a cryocooled crystal. (b) Diffraction image from room-temperature data collection. The deleterious effects of the cryoprotectant are evident in the diffuse scattering and increase in the mosaic spread (left). Reflections in the room-temperature diffraction data set are well defined, separated and appear to have a low mosaic spread.
