Table 1.
Summary of statistical results.
| Data set | Statistic used | df | F or t value | Sig. level | Figure |
|---|---|---|---|---|---|
| CPP | 1 | ||||
| FN439-Nic vs. aCSF-Nic | Indep t-test | 9 | 4.16 | P < 0.01 | |
| HIP MMP-2 | 2A | ||||
| aCSF-PBS vs. Naïve HCC | 2 × 5 ANOVA | ||||
| Groups | 1,38 | 18.44 | P < 0.001 | ||
| Days | 4,38 | 169.68 | P < 0.001 | ||
| Grps × days | 4,38 | 9.45 | P < 0.005 | ||
| aCSF-Nic vs. Naïve HCC | 2 × 5 ANOVA | ||||
| Groups | 1,39 | 20.92 | P < 0.001 | ||
| Days | 4,39 | 129.02 | P < 0.001 | ||
| Grps × days | 4,39 | 8.17 | P < 0.005 | ||
| HIP MMP-3 | 2A | ||||
| aCSF-PBS vs. Naïve HCC | 2 × 5 ANOVA | ||||
| Groups | 1,38 | – | Not sig. | ||
| Days | 4,38 | 96.96 | P < 0.001 | ||
| Grps × days | 4,38 | 9.07 | P < 0.005 | ||
| aCSF-Nic vs. Naïve HCC | 2 × 5 ANOVA | ||||
| Groups | 1,41 | – | Not sig. | ||
| Days | 4,41 | 72.31 | P < 0.001 | ||
| Grps × days | 4,41 | 12.74 | P < 0.001 | ||
| HIP MMP-9 | 2A | ||||
| aCSF-PBS vs. Naïve HCC | 2 × 5 ANOVA | ||||
| Groups | 1,40 | 21.65 | P < 0.001 | ||
| Days | 4,40 | 79.70 | P < 0.001 | ||
| Grps × days | 4,40 | 8.84 | P < 0.005 | ||
| aCSF-Nic vs. Naïve HCC | 2 × 5 ANOVA | ||||
| Groups | 1,45 | 22.47 | P < 0.001 | ||
| Days | 4,45 | 12.04 | P < 0.001 | ||
| Grps × days | 4,45 | 6.85 | P < 0.005 | ||
| PFC MMP-2 | 3A | ||||
| aCSF-PBS vs. Naïve HCC | 2 × 5 ANOVA | ||||
| Groups | 1,26 | 14.26 | P < 0.001 | ||
| Days | 4,26 | 170.67 | P < 0.001 | ||
| Grps × days | 4,26 | 12.50 | P < 0.001 | ||
| aCSF-Nic vs. Naïve HCC | 2 × 5 ANOVA | ||||
| Groups | 1,26 | 18.93 | P < 0.001 | ||
| Days | 4,26 | 122.24 | P < 0.001 | ||
| Grps × days | 4,26 | 9.40 | P < 0.005 | ||
| PFC MMP-3 | 3A | ||||
| aCSF-PBS vs. Naïve HCC | 2 × 5 ANOVA | ||||
| Groups | 1,35 | 11.92 | P < 0.001 | ||
| Days | 4,35 | 248.69 | P < 0.001 | ||
| Grps × days | 4,35 | 4.35 | P < 0.01 | ||
| aCSF-Nic vs. Naïve HCC | 2 × 5 ANOVA | ||||
| Groups | 1,38 | 20.65 | P < 0.001 | ||
| Days | 4,38 | 198.07 | P < 0.001 | ||
| Grps × days | 4,38 | 4.48 | P < 0.005 | ||
| PFC MMP-9 | 3A | ||||
| aCSF-PBS vs. Naïve HCC | 2 × 5 ANOVA | ||||
| Groups | 1,38 | – | Not sig. | ||
| Days | 4,38 | 61.55 | P < 0.001 | ||
| Grps × days | 4,38 | – | Not sig. | ||
| aCSF-Nic vs. Naïve HCC | 2 × 5 ANOVA | ||||
| Groups | 1,40 | – | Not sig. | ||
| Days | 4,40 | 232/48 | P < 0.001 | ||
| Grps × days | 4,40 | 8.36 | P < 0.001 | ||
| HIP cortactin | 4A | ||||
| aCSF-PBS vs. Naïve HCC | 2 × 5 ANOVA | ||||
| Groups | 1,40 | – | Not sig. | ||
| Days | 4,40 | 8.60 | P < 0.001 | ||
| Grps × days | 4,40 | 11.43 | P < 0.001 | ||
| aCSF-Nic vs. Naïve HCC | 2 × 5 ANOVA | ||||
| Groups | 1,38 | – | Not sig. | ||
| Days | 4,38 | 9.59 | P < 0.001 | ||
| Grps × days | 4,38 | 3.81 | P < 0.02 | ||
| PFC cortactin | 5A | ||||
| aCSF-PBS vs. Naïve HCC | 2 × 5 ANOVA | ||||
| Groups | 1,35 | – | Not sig. | ||
| Days | 4,35 | – | Not sig. | ||
| Grps × days | 4,35 | 17.91 | P < 0.001 | ||
| aCSF-Nic vs. Naïve HCC | 2 × 5 ANOVA | ||||
| Groups | 1,38 | – | Not sig. | ||
| Days | 4,38 | – | Not sig. | ||
| Grps × days | 4,38 | 25.28 | P < 0.001 | ||
| CPP: aCSF-Nic | 6 | ||||
| Init. pref. vs. Post-cond. | Paired t-test | 4 | 5.75 | P < 0.01 | |
| Init. pref. vs. Re-exp | Paired t-test | 4 | 8.65 | P < 0.001 | |
| Post-cond. vs. Re-exp | Paired t-test | 4 | 5.32 | P < 0.01 | |
| HIP Re-Exp. MMP-3 | 7A | ||||
| aCSF-PBS vs. naïve HCC | Indep t-test | 10 | 7.75 | P < 0.001 | |
| aCSF-Nic vs. naïve HCC | Indep t-test | 10 | 7.21 | P < 0.001 | |
| PFC Re-Exp. MMP-3 | 8B | ||||
| aCSF-PBS vs. naïve HCC | Indep t-test | 10 | 12.22 | P < 0.001 | |
| aCSF-Nic vs. naïve HCC | Indep t-test | 10 | 11.89 | P < 0.001 |
Abbreviations: aCSF, artificial cerebrospinal fluid; ANOVA, analysis of variance; cond, conditioning; CPP, conditioned place preference; df, degrees of freedom; exp, exposure; grps, groups; HCC, home cage control; HIP, hippocampus; indep, independent; init, initial; MMP, matrix metalloproteinase; Nic, nicotine; P, probability; PBS, phosphate buffered saline; PFC, prefrontal cortex; sig, significance (or significant); vs., versus.