Table 9. Multilocus AMOVA analysis of TcI T. cruzi subpopulations from Brazil.

Source of variation	df	Sum of squares	Variance componentsb	Percentage of variation
Among populationsa	2	6.847	0.21309Va	16.98
Within populations	31	32.300	1.04194Vb	83.02
Total	33	39.147	1.25503
Fixation index	FST	0.16979

^aPopulations: TcI Brazilian subpopulations TcI-Br-A, TcI-Br-B and TcI-Br-C (Table 3). Distance: number of different alleles (F_ST) (see the section on ‘Materials and methods’).

Significance tests (1023 permutations):

V_a and F_ST: P(rand. value>obs. value) = 0.00196

P(rand. value = obs. value) = 0.00000

P(rand. value> = obs. value) = 0.00196±0.00136.