Table 2. Observed proportions of various families of msp-1 and msp-2, allele numbers and size range among P. falciparum isolates of Ranchi, Hazaribag, Bokaro, Palamau and Latehar districts of Jharkhand.
Ranchi | Hazaribag | Bokaro | Palamau | Latehar | ||
Gene | Genotype | |||||
Pfmsp-1 | ||||||
K1 | ||||||
Observed nos. | 13 | 15 | 13 | 10 | 12 | |
Observed allele nos. | 4 | 5 | 3 | 7 | 6 | |
Size range (bp) | 160–400 | 160–350 | 170–300 | 180–400 | 160–400 | |
MAD20 | ||||||
Observed nos. | 16 | 20 | 14 | 17 | 18 | |
Observed allele nos. | 5 | 9 | 6 | 8 | 7 | |
Size range (bp) | 175–500 | 180–600 | 175–400 | 175–550 | 175–600 | |
RO33 | ||||||
Observed nos. | 7 | 8 | 10 | 5 | 8 | |
Observed allele nos. | 2 | 1 | 2 | 3 | 2 | |
Size range (bp) | 170, 220 | 220 | 170, 220 | 170, 220, 200 | 170, 220 | |
Proportion of unique alleles | 11/36 = 30.5% | 15/43 = 34.8% | 11/37 = 29.7% | 18/32 = 56.2% | 15/38 = 39.4% | |
Multiplicity of infection | 1.44 | 1.43 | 1.42 | 1.28 | 1.35 | |
Pfmsp-2 | ||||||
FC27 | ||||||
Observed nos. | 11 | 19 | 15 | 15 | 18 | |
Observed allele nos. | 7 | 4 | 5 | 8 | 9 | |
Size range (bp) | 240–550 | 250–600 | 240–500 | 250–550 | 250–600 | |
3D7 | ||||||
Observed nos. | 20 | 23 | 22 | 22 | 22 | |
Observed allele nos. | 9 | 3 | 5 | 8 | 11 | |
Size range (bp) | 450–600 | 460–650 | 450–550 | 450–650 | 460–600 | |
Proportion of unique alleles | 16/31 = 51.6% | 7/42 = 16.6% | 10/37–27% | 16/37 = 43.2% | 20/40 = 50% | |
Multiplicity of infection | 1.24 | 1.4 | 1.42 | 1.48 | 1.42 |