Table 1.

Identification of relevant biological functions and canonical pathways through the use of IPA.

	MOLECULES	B-H P-VALUE	RATIO
Ingenuity Biological Functions
Nervous System Development and Function	DPYSL2, GDA, MAPK1, DPYSL3, PDIA3, DPYSL4, FAS, STMN1, ACTR3, SOD2, TARDBP, PURA, MAP2K1, CFL1, CASP3, ACTB, TKT, GNAQ, CKMT1A/CKMT1B, DPYSL5, DNM1, HSPA8, CRMP1, STMN3, GNAO1, GAP43, DNM1L, SYN2	1.73E-02–7.77E-02	/
Ingenuity Canonical Pathways
Protein Ubiquitination Pathway	PSMB4, PSMA7, HSPH1, THOP1, PSMA1, DNAJA1, HSPA8, PSMB2, PSMB1, PSMA4, UBA1, PSMA2, PSMC2, HSPA4L	6.23E-06	14/268 = 0.0522
Semaphorin Signaling in Neurons	DPYSL2, CRMP1, MAPK1, CFL1, DPYSL3, DPYSL4 DPYSL5	1.82E-05	7/52 = 0.135

IPA-analysis (www.ingenuity.com) was used to identify adaptations in processes related to nervous system development and function, protein ubiquitination pathway, and semaphorin signaling in neurons play an important role in mouse forebrain postnatal development. The significance of the biological functions and canonical pathways were tested by the stringent Benjamini-Hochberg (B-H) multiple testing correction method. The ratio value indicates the number of differential proteins in a given pathway divided by the total number of molecules that make up that pathway. The following proteins/molecules, annotated with their gene names, were included in the biological functions and canonical pathways. Proteins from the protein ubiquitination pathway and semaphorin signaling in neurons selected for ISH validation are indicated in bold.

Abbreviations: DPYSL2 (or CRMP2), dihydropyrimidinase-like 2; GDA, guanine deaminase; MAPK1, mitogen-activated protein kinase 1; DPYSL3 (or CRMP4), dihydropyrimidinase-like 3; PDIA3, protein disulfide isomerase family A, member 3; DPYSL4 (or CRMP3), dihydropyrimidinase-like 4; FAS, Fas (TNF receptor superfamily, member 6); STMN1, stathmin 1; ACTR3, ARP3 actin-related protein 3 homolog; SOD2, superoxide dismutase 2, mitochondrial; TARDBP, TARDNA binding protein; PURA, purine-rich element binding protein; MAP2K1, mitogen-activated protein kinase kinase 1; CFL1, cofilin 1 (non-muscle); CASP3, caspase3, apoptosis-related cysteine peptidase; ACTB, actin, beta; TKT, transketolase; GNAQ, guanine nucleotide binding protein (G protein), q polypeptide; CKMT1A/CKMT1B, creatine kinase, mitochondrial 1B; DPYSL5 (or CRMP5), dihydropyrimidinase-like 5; DNM1, dynamin 1; HSPA8, heat shock 70kDa protein 8; CRMP1, collapsin response mediator protein 1; STMN3, stathmin 3; GNAO1, guanine nucleotide binding protein (G protein), alpha activating activity polypeptide O; GAP43, growth associated protein 43; DNM1L, dynamin 1-like; SYN2, synapsin II; PSMB4, proteasome (prosome, macropain) subunit, beta type, 4; PSMA7, proteasome (prosome, macropain) subunit, alpha type, 7; HSPH1, heat shock 105kDa/110kDa protein 1; THOP1, thimet oligopeptidase 1; PSMA1, proteasome (prosome, macropain) subunit, alpha type, 1; DNAJA1, DnaJ (Hsp40) homolog, subfamily A, member 1; PSMB2, proteasome (prosome, macropain) subunit, beta type, 2; PSMB1, proteasome (prosome, macropain) subunit, beta type, 1; PSMA4, proteasome (prosome, macropain) subunit, alpha type, 4; UBA1, ubiquitin-like modifier activating enzyme 1; PSMA2, proteasome (prosome, macropain) subunit, alpha type, 2; PSMC2, proteasome (prosome, macropain) 26S subunit, ATPase, 2; HSPA4L, heat shock 70kDa protein 4-like.