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. 2014 Jun 12;14:424. doi: 10.1186/1471-2407-14-424

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Copyright © 2014 Song et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Present of Fli-1 and growth characteristics of SKOV3 cells with Fli-1 expression down-regulated. (a) Western blots showing the purity of the isolated nucleus/cytoplasm sample, nuclear (N) and cytoplasmic (C). (b) SiRNA transfection efficiency in tumor cells was measured by Western blotting; (c) Transwell migration assay of the indicated cell lines transfected with Fli-1 constructs or transient transfected with two different Fli-1 siRNA target sequence. (d) The growth curve displays the absolute counts of cells cultured in twelve-well plates during the 4-day treatment.