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. Author manuscript; available in PMC: 2014 Jul 9.
Published in final edited form as: J Nat Prod. 2012 Feb 16;75(4):591–598. doi: 10.1021/np200823p

Figure 1.

Figure 1

Bryonolic acid (BA) (1) decreases NO levels and inhibits iNOS expression in RAW 264.7 cells in a dose dependent and time dependent manner. RAW 264.7 cells were activated with 5 ng/mL LPS and treated with varying concentrations of BA for 24 hours (A-D) or varying time points (E). (A) Nitrite levels were measured via Griess assay in LPS-activated cells treated with BA for 24 hours. (B) RAW 264.7 cells were treated with varying concentrations of BA for 24 hours and the viability measured by the MTT assay. (C) iNOS protein levels were quantified through immunoblot analysis in LPS-activated cells treated with varying concentrations of BA for 24 hours. (D) iNOS mRNA levels were measured by RT-PCR. (E) iNOS protein levels were quantified through immunoblot analysis in LPS-activated cells treated with 100 μM BA or DMSO control at various time points.