Figure 2.
Bryonolic acid (BA) (1) induces HO-1 expression in RAW 264.7 cells in an LPS independent manner. RAW 264.7 cells were either activated with 5 ng/mL LPS (A-B) or without (C-D) with varying concentrations of BA for 24 hours (A-D) or varying time points (E). (A, C and E) Immunoblot analysis of HO-1 protein levels. (B and D) mRNA level measurement of HO-1 by RT-PCR.